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青島雙元泰和藥業有限公司

青島市城陽區雙元路218號

電話: 0532-55676230
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福建快三2月推荐:疏水肽


谁有大量微信群卖 www.pucvo.com 雙元泰和疏水性多肽合成基于專有的技術平臺,為您提供研究級的定制多肽和疏水肽。

什么是疏水肽技術


雙元泰和疏水肽技術包括:

  1. 混合合成平臺: 固相谁有大量微信群卖(SPPS)/ 液相多肽合成 (LPPS), 連接技術和裂碎技術
  2. 聯合純化平臺:
    • 凝膠滲透色譜(GPC)
    • 離子交換色譜(IEC)
    • 反相高效液相色譜(RP-HPLC)
    疏水肽技術改善合成效率,下圖描述了合成過程: HydroFobic Peptide technology

    為什么開發疏水肽技術?

    單個蛋白質的3D結構以及生物活性主要由其組分氨基酸的一級序列決定。肽鏈形成有序結構的固有傾向是在肽組裝期間遇到的合成效率的高度序列特異性變異性的主要原因。由于肽是細長的,它可以與其他肽鏈或聚合物載體形成二級結構或聚集體,這可導致較低的反應速率。該效應可以僅從微妙的減速到脫?;ず王;從Φ耐耆?。在這種極端情況下,多肽變得不溶,不再可用于反應。給定序列的空間結構通常難以預測,盡管含有連續疏水性氨基酸的片段如Ala,Val,Ile以及含有可以形成鏈內氫鍵氨基酸(如Gln和Thr)的多肽常常難以制備,這是使多肽合成有趣和具有挑戰性的因素之一。因此,一般來說,最好從開始的合成策略中采用減輕結構形成的方案,而不是嘗試第二次猜測哪些肽可能是有問題的而浪費時間和資源重復失敗的合成。為此,雙元泰和開發了疏水肽技術,解決了大部分問題。

    多肽為什么合成困難?

    描述 解釋
    Multiple Aspartic Acids (D) Multiple Aspartic Acids (D) in a sequence frequently result in the formation of aspartimide adducts, resulting in a product of lower purity.
    Adjacent Serines (S) Adjacent Serines (-AA-S-) in a sequence frequently result in product that is low in purity and/or contain many deletions
    Poly(GGGG) Multiple consecutive Glycines (G) (4 or more) tend to undergo hydrogen bonding (gel formation) in the peptide backbone. The hydrogen bonding may cause difficulty in dissolving and purifying the peptide
    Difficult amino acids: C, M, W Especially in summer, it's easily to oxidation if peptide containing C or M or W
    >75% hydrophobic residues, and contains 25% charged residues May be insoluble or only partially soluble in aqueous solutions
    >50% hydrophobic residues, and contains 25% negative charged residues May be insoluble or only partially soluble in aqueous solutions
    >50% to 75% hydrophobic residues and without charged residues May be insoluble or only partially soluble in aqueous solutions
    Very hydrophobic 谁有大量微信群卖 containing >75% hydrophobic residues Generally not dissolve in aqueous solutions
    Secondary Structure: The peptide containing streches of contiguous hydrophobic amino acids: Val, Ile, Ala as well as those containing amino acids wich can form intra-chain or inter-chain hydrogen bonds, such as Gln or Thr. During synthesis, ?-sheet formation causes incomplete solvation of the growing peptide and results in a high degree of deletion sequences in the final product.